Research notes, musings, and writing from James LaFemina.https://www.jameslafemina.com/https://www.jameslafemina.com/protocols/cortical-organoid-protocol-guide/https://www.jameslafemina.com/protocols/cortical-organoid-protocol-guide/Step-by-step protocol for generating human cortical organoids from iPSCs.Sun, 08 Mar 2026 00:00:00 GMTorganoidscorticalprotocolhttps://www.jameslafemina.com/protocols/thalamic-organoid-protocol-guide/https://www.jameslafemina.com/protocols/thalamic-organoid-protocol-guide/Step-by-step protocol for generating human thalamic organoids from iPSCs.Sun, 08 Mar 2026 00:00:00 GMTorganoidsthalamicprotocolhttps://www.jameslafemina.com/protocols/midbrain-organoid-protocol-guide/https://www.jameslafemina.com/protocols/midbrain-organoid-protocol-guide/Step-by-step protocol for generating human midbrain organoids from iPSCs.Sat, 28 Feb 2026 00:00:00 GMTorganoidsmidbrainprotocolhttps://www.jameslafemina.com/research/dopamine-research-proposal/https://www.jameslafemina.com/research/dopamine-research-proposal/Research proposal exploring dopaminergic dynamics in midbrain organoid models.Thu, 26 Feb 2026 00:00:00 GMTdopaminemidbrainresearch-proposalparkinsonshttps://www.jameslafemina.com/protocols/rotenone-dose-calibration/https://www.jameslafemina.com/protocols/rotenone-dose-calibration/Protocol for calibrating rotenone dose-response in midbrain organoid models.Mon, 26 Jan 2026 00:00:00 GMTrotenonedose-calibrationorganoidsparkinsonsprotocolhttps://www.jameslafemina.com/musings/my-story/https://www.jameslafemina.com/musings/my-story/A brief history of me and my relationship to life.Mon, 24 Nov 2025 00:57:00 GMThttps://www.jameslafemina.com/musings/statement_of_purpose/https://www.jameslafemina.com/musings/statement_of_purpose/A brief history of me and my relationship to life.Mon, 24 Nov 2025 00:57:00 GMThttps://www.jameslafemina.com/research/adaptive-burst-detection/https://www.jameslafemina.com/research/adaptive-burst-detection/Developing threshold methods that adapt to individual organoid characteristics rather than using fixed parametersThu, 20 Nov 2025 00:00:00 GMTelectrophysiologyburst-analysisorganoidsparkinson'shttps://www.jameslafemina.com/research/braingeneers_presentation_oct2025/https://www.jameslafemina.com/research/braingeneers_presentation_oct2025/Talk on adaptive burst detection in midbrain organoids.Wed, 15 Oct 2025 00:00:00 GMTpresentationorganoidsburst-analysis